Shake the sample container briefly. Place the tube of the pipette into the fuel sample and squeeze the bulb. Release the bulb of the pipette. This should fill the tube with fuel. Repeat this process if necessary, until the tube of the pipette is full. The small chamber under the bulb should not contain any sample. Avoid over filling the pipette as it will lead to incorrect volumes being dispensed.
Lay the bottle flat, with the gel layer down, in a warm location; the optimum temperature range is 22 – 28 °C (72 – 82 °F). If the temperatures are slightly lower, the results may take longer to develop. Examine the test daily for the next 1 to 4 days. Avoid shaking the test bottle during this time.
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Use biocides safely. Always read the label and product information before use.